A Transcriptional Analysis Showing the Effects of GH12 Combined with Fluoride for Suppressing the Acidogenicity of Streptococcus mutans Biofilms

The acidogenicity of Streptococcus mutans is important for caries development. antimicrobial peptide GH12 can affect the integrity cellular membranes and virulence factors S. mutans. Combining NaF (GF) efficiently controlled development caries, but its mechanisms remained unrevealed. This research intended to verify effects GF on biofilms reveal mechanisms. Lactic acid production assays pH monitoring were conducted investigate regulatory treatment biofilms. RNA sequencing bioinformatics analyses screen transcriptional profile affected by treatment. results demonstrated group had significantly less lactic maintained broth’s values above 5.0 longer times. Thereafter, GO/KEGG enrichment RT-qPCR validation revealed that mainly restrained expression genes related carbohydrates’ internalization metabolism. Compared with NaF, further downregulated carbohydrates transportation genes. Moreover, compared GH12, membrane’s more significantly. Generally, could arrest biofilms, through suppressing inhibiting overall